HIGHLY EFFICIENT EXPRESSION OF FISH GROWTH-HORMONE BY ESCHERICHIA-COLI-CELLS

Citation
Hj. Tsai et al., HIGHLY EFFICIENT EXPRESSION OF FISH GROWTH-HORMONE BY ESCHERICHIA-COLI-CELLS, Applied and environmental microbiology, 61(11), 1995, pp. 4116-4119
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
61
Issue
11
Year of publication
1995
Pages
4116 - 4119
Database
ISI
SICI code
0099-2240(1995)61:11<4116:HEEOFG>2.0.ZU;2-Z
Abstract
A PCR product encoding the mature segment of fish pregrowth hormone (p re-GH) was inserted into an Escherichia coli expression vector, pET, i n which the ori site was replaced by that of pUC19. The yield of recom binant GH (rGH) was as high as 44 to 47% of total protein. This rGH wa s immunoreactive to GH antibody, After renaturation, rGH was used to i nject fish with 0.1 mu g of rGH per g once every 2 weeks, and this res ulted in increases in weight (65%), percent weight gain (165%), and le ngth (22%) relative to those of an untreated control group at week 16 and onward.