THE GOLGI-COMPLEX OF SACCHAROMYCES-CEREVISIAE

The Golgi complex of Saccharomyces cerevisae is required for protein t ransport, posttranslational modification, and sorting within the secre tory pathway. Only in the last few years has it become clear that thes e events are functionally organized into distinct Golgi compartments a nalagous to the cis-trans compartments of the plant and animal Golgi c omplex. The mechanisms by which the Golgi complex maintains its own or ganization and complement of intrinsic enzymes against the flow of lip id and protein through the secretory pathway are still poorly understo od. However, studies using Saccharomyces cerevisiae as a model system are providing insights into mechanisms of Golgi protein localization t hat appear to be conserved between yeast and mammalian cells. This rev iew describes the structure and organization of the yeast Golgi comple x and recent work towards defining localization signals within intrins ic enzymes of this organelle.