DETERMINATION OF PENTACHLOROPHENOL IN WATER AND SOIL BY A MAGNETIC PARTICLE-BASED ENZYME-IMMUNOASSAY

A competitive enzyme immunoassay using specific pentachlorophenol (PCP ) antiserum covalently coupled to a magnetic particle solid phase has been developed for the detection of PCP in water and soil. The immunoa ssay allows the quantification of PCP from 100 ppt (parts per trillion , ng/L) in water and 100 ppb (parts per billion, mg/L) in soil. Spike recovery from water samples with no sample pretreatment and from vario us soil types following a simple extraction technique averaged 105% an d 94%, respectively. The method compares favorably with GC/MS and HPLC measurements in water (r = 0.980) and soil (r = 0.996) samples.