Cs. Hottenstein et al., DETERMINATION OF PENTACHLOROPHENOL IN WATER AND SOIL BY A MAGNETIC PARTICLE-BASED ENZYME-IMMUNOASSAY, Environmental science & technology, 29(11), 1995, pp. 2754-2758
A competitive enzyme immunoassay using specific pentachlorophenol (PCP
) antiserum covalently coupled to a magnetic particle solid phase has
been developed for the detection of PCP in water and soil. The immunoa
ssay allows the quantification of PCP from 100 ppt (parts per trillion
, ng/L) in water and 100 ppb (parts per billion, mg/L) in soil. Spike
recovery from water samples with no sample pretreatment and from vario
us soil types following a simple extraction technique averaged 105% an
d 94%, respectively. The method compares favorably with GC/MS and HPLC
measurements in water (r = 0.980) and soil (r = 0.996) samples.