CHANGES IN LYMPHOPROLIFERATION AND DTH RESPONSES AFTER VACCINATION IMMEDIATELY BEFORE SURGERY IN PUPPIES

To clarify the effects of the presurgical vaccination with inactivated canine parvovirus vaccine (CPV) on immunological responses to surgery in puppies, we assessed it by measuring the blastogenic responses of lymphocytes and delayed type hypersensitivity (DTH) responses after la parotomy in the non- and vaccinated puppies. The inhalation anesthetic used was isoflurane or halothane. In post-surgery, the blastogenic re sponses of lymphocytes in the non-vaccinated puppies decreased, especi ally, those in these puppies with halothane aneshesia (GOF) did signif icantly, and the duration of this decrease prolonged more than that in the non-vaccinated puppies with isoflurane anesthesia (GOI). However, the responses in the vaccinated puppies with each anesthesia didn't a lmost decrease below the presurgical levels. In GOI groups, the DTH re sponses in vaccinated puppies increased significantly over those in no n-vaccinated ones, but in GOF groups, there were few differences betwe en the DTH responses in the non- and vaccinated puppies. These results suggest that the CPV vaccination immediately before surgery possibly prevents the postsurgical immunosuppression, and that GOI may depress the immunocompetence less than GOF.