A CHROMOSOME-SPECIFIC MICRODISSECTED LIBRARY INCREASES MARKER DENSITYON BOVINE CHROMOSOME-1

Authors
SONSTEGARD TS DELEON FAP BEATTIE CW KAPPES SM
Citation
Ts. Sonstegard et al., A CHROMOSOME-SPECIFIC MICRODISSECTED LIBRARY INCREASES MARKER DENSITYON BOVINE CHROMOSOME-1, PCR methods and applications, 7(1), 1997, pp. 76-80
Citations number
27
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
PCR methods and applications → ACNP
ISSN journal
10549803
Volume
7
Issue
1
Year of publication
1997
Pages
76 - 80
Database
ISI
SICI code
1054-9803(1997)7:1<76:ACMLIM>2.0.ZU;2-E
Abstract
Genetic resolution of bovine chromosome 1 (BTA1) linkage group was sig nificantly increased by screening for microsatellite clones in a micro dissected library constructed from a bovine cell line carrying a t(1;2 9) translocation. Eighty-five percent of the microsatellites (ms) (46/ 54) identified were informative in the USDA/MARC mapping population, a nd 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged wit h 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-avera ged interval, 1.9 cM). The fourfold improvement in marker density of B TA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.