HIGH-THROUGHPUT MICROSATELLITE ANALYSIS USING FLUORESCENT DUTPS FOR HIGH-RESOLUTION GENETIC-MAPPING OF THE MOUSE GENOME

The use of fluorescent end-labeled primers has proved successful for r apid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs call be prohibitive when a wide rang e of markers is to be analyzed for only a few genotypings. This partic ularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high- resolution genetic maps For positional cloning. We demonstrate here th e use of fluorescent dUTPs for cost-effective, high-throughput microsa tellite genotyping in the mouse. This alternative to the use of fluore scent end-labeled primers For semiautomated genotyping is potentially applicable to the construction of linkage maps in other species.