APPROACHES TO DETECTION OF DISTANTLY RELATED PROTEINS BY DATABASE SEARCHES

Authors
CATTELL K KOOP B OLAFSON RS FELLOWS M BAILEY I OLAFSON RW UPTON C
Citation
K. Cattell et al., APPROACHES TO DETECTION OF DISTANTLY RELATED PROTEINS BY DATABASE SEARCHES, BioTechniques, 21(6), 1996, pp. 1118
Citations number
14
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
21
Issue
6
Year of publication
1996
Database
ISI
SICI code
0736-6205(1996)21:6<1118:ATDODR>2.0.ZU;2-P
Abstract
The searching of protein databases as a method of identifying newly se quenced genes is commonplace in molecular biology laboratories. Howeve r, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly. This article uses a sing le family of highly diverged uracil-DNA glycosylases, which fall into two distinct groups, to highlight some of the difficulties associated with identification of such proteins by database searching.