CDNA CLONING OF PORCINE GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR

Porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) cDNA was cloned by using polymerase chain reaction (PCR) and sequenced. A coding sequence of the porcine GM-CSF precursor protein, including the signal peptide sequence and stop codon, is 435 bp in length. The iden tities of the porcine GM-CSF coding sequence when compared to ovine, b ovine, human and murine sequences were 89, 86, 83 and 70% at the nucle otide level, and 80, 74, 73 and 56% at the amino acid level. The hydro phobicity profiles, putative glycosylation sites and positions of cyst eine residues were highly conserved in porcine, ovine, bovine and huma n GM-CSF but not murine. This is the first report of the porcine GM-CS F cDNA cloning and sequence.