RATE PARAMETERS AND GRADIENT CORRELATIONS FOR GRADIENT-ELUTION CHROMATOGRAPHY

Rate parameters and gradient correlations of proteins are very importa nt to engineering investigations of gradient-elution chromatography. B efore carrying out computer simulations for gradient process, these ra te parameters and gradient correlations must be determined from experi mental data. This work presents a systematic method for estimating and determining these parameters and correlations using experimental data and computer-simulation results based on isocratic runs. The values d etermined were then employed in gradient-elution studies by putting th em into the rate model to perform computer simulations. Experimental r uns under gradient conditions using the parameters and correlations ju st referred to were carrier out in a DEAE Sepharose CL-6B ion-exchange chromatography. Comparisons between the experimental data and the sim ulation results show good agreement.