J. Teichert et R. Preiss, DETERMINATION OF LIPOIC ACID IN HUMAN PLASMA BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Journal of chromatography B. Biomedical applications, 672(2), 1995, pp. 277-281
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A selective and sensitive method for the determination of lipoic acid
in human plasma samples has been developed. After enzymatic hydrolysis
of the sample, the liberated lipoic acid was extracted by a solid-pha
se cartridge and measured by HPLC using electrochemical detection. The
detection limit was 1 ng/ml lipoic acid in plasma. The calibration cu
rve was non-linear in the range 0.01-50 mu g/ml but could be described
by a power function. The average extraction recoveries were 82.5 and
85.1% at the 25 and 2500 ng/ml levels, respectively. Coefficients of v
ariation for both within-day and day-to-day analysis were between 2.1
and 9.4%. The assay method is sensitive, reproducible and suitable for
disposition studies of lipoic acid in humans.