HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF FLUORESCENT ESTERS OF HEPOXILIN ENANTIOMERS ON A CHIRAL STATIONARY-PHASE

Fluorescent anthryl (ADAM) derivatives of hepoxilins have been shown t o possess good chromatographic properties affording good sensitivity f or the high-performance liquid chromatographic analysis and detection of these compounds and related eicosanoids (12-hydroxyeicosatetraenoic acid) in biological samples, We report herein the separation of all p ossible stereoisomers of hepoxilins A(3) and B-3 as their methyl ester s as well as their ADAM ester and acetate derivatives on a cellulose t risdimethylphenylcarbamate chiral stationary phase (Chiracel OD) in th e normal-phase mode. This methodology is important to address the mech anistic route of biosynthesis of these products.