RMP-7, A BRADYKININ AGONIST, INCREASES PERMEABILITY OF BLOOD-OCULAR BARRIERS IN THE GUINEA-PIG

Purpose. To determine if the bradykinin agonist, RMP-7, could increase the ocular tissue concentration of agents that normally have limited access across the blood-ocular barriers. The extracellular space marke r, sucrose, and the anti-viral drug, ganciclovir, were tested. Methods . Using the perfused eye method in guinea pigs, RMP-7 (1 mu g/kg over 5 minutes) or saline were administered intraarterially into the ocular circulation before either radiolabeled sucrose or ganciclovir (0.4 to 0.6 mu Ci/ml per minute). At time intervals ranging from 0.25 minute to 10 minutes, perfused eyes were removed, and the radioactivity withi n various compartments was measured using liquid scintillography. Resu lts. Pretreatment with RMP-7 significantly increased uptake of both su crose (up to 4.5-fold) and ganciclovir (up to 2-fold) into the guinea pig retina and lens. Smaller and less consistent effects were observed in other eye compartments. Conclusions. This report demonstrates that RMP-7 enhances the permeability of blood-ocular barriers, and it prov ides the first pharmacologic evidence for a means to enhance the conce ntration of ganciclovir into the retina. Thus, these data support the concept that RMP-7 may prove to be a useful adjunct for enhanced deliv ery of therapeutics to the eye under conditions in which ocular barrie rs limit treatment.