CONSTRUCTION OF A DIVALENT CELL ADHESIVE LYSOZYME BY INTRODUCING THE ARG-GLY-ASP SEQUENCE AT 2 SITES

To increase the cell adhesion activity of 74RGD4, an RGDS-inserted mut ant between Val(74) and Asn(75) of human lysozyme, one more site for t he RGD introduction was investigated in the lysozyme molecule, We foun d that 47RGD4 with RGDS in place of AGDR (residues 47 to 50) in a beta -turn region possesses the same level of adhesion activity as that of 74RGD4, The acceptance of the RGD introduction in the beta-turn region of human lysozyme is in good agreement with recent studies on the fun ctional conformation of RGD, We constructed (47,74)RGD4, a mutant cont aining RGD at two sites, by combining the N-terminal domain of 47RGD4 and the C-terminal domain of 74RGD4, The (47,74)RGD4 lysozyme, with tw o functional RGD sequences, exhibits even higher cell adhesion activit y than that of 74RGD4 or 47RGD4.