T. Yamada et al., CONSTRUCTION OF A DIVALENT CELL ADHESIVE LYSOZYME BY INTRODUCING THE ARG-GLY-ASP SEQUENCE AT 2 SITES, FEBS letters, 374(2), 1995, pp. 262-264
To increase the cell adhesion activity of 74RGD4, an RGDS-inserted mut
ant between Val(74) and Asn(75) of human lysozyme, one more site for t
he RGD introduction was investigated in the lysozyme molecule, We foun
d that 47RGD4 with RGDS in place of AGDR (residues 47 to 50) in a beta
-turn region possesses the same level of adhesion activity as that of
74RGD4, The acceptance of the RGD introduction in the beta-turn region
of human lysozyme is in good agreement with recent studies on the fun
ctional conformation of RGD, We constructed (47,74)RGD4, a mutant cont
aining RGD at two sites, by combining the N-terminal domain of 47RGD4
and the C-terminal domain of 74RGD4, The (47,74)RGD4 lysozyme, with tw
o functional RGD sequences, exhibits even higher cell adhesion activit
y than that of 74RGD4 or 47RGD4.