SPECTROFLUOROMETRIC DETERMINATION OF CHLORPROMAZINE HYDROCHLORIDE ANDTHIORIDAZINE HYDROCHLORIDE

Fluorescence spectroscopy was applied to the development of a sensitiv e and simple method for the determination of chlorpromazine HCl and th ioridazine-HCl. The method is based upon development of an intense flu orescence using N-bromosuccinimide as the fluorogenic reagent. The pro duced fluorescence has very characteristic excitation and emission spe ctra and was stable for at least one hour. The results were reproducib le and as little as 5 ng/ml chloropromazine HCl and 1 ng/ml thioridazi ne-HCl could be determined. The method was applied successfully to the analysis of various commercially available dosage forms. The obtained results were in good agreement with those of the official BP 93 proce dures.