CARBON-DIOXIDE SUPERCRITICAL-FLUID EXTRACTION WITH ONLINE FLUORESCENCE DETECTION

An HPLC detector was modified to operate as a low pressure on-line sup ercritical fluid extraction detector. Spectra of CO2 and fluorene are presented of pressures ranging from 85 to 150 atm. Bath the intensity and emission wavelength of fluorene were effected by CO2 pressure. As pressure increased, the emission intensity also increased and the emis sion shifted to a shorter wavelength. The intensity of CO2 emission de creased by increasing pressure. The emission wavelength of CO2 was una ffected by pressure. A detection limit for fluorene was found to be 5 mu g per extraction. The minimal detectable mass was 25 mu g (+/- 8% R SD) per extraction.