Mt. Colomina et al., EFFECTS OF MATERNAL STRESS ON METHYLMERCURY-INDUCED DEVELOPMENTAL TOXICITY IN MICE, Physiology & behavior, 58(5), 1995, pp. 979-983
The developmental toxicity of combined exposure to maternal restraint
stress and methylmercury chloride (MMC) was assessed in Swiss mice. On
day 10 of gestation, four groups of plug-positive female mice were tr
eated (p.o.) with a single dose of 12.5 or 25 mg MMC/kg. Immediately a
fter MMC exposure, two of those groups were subjected to restraint for
14 hr. Control groups included restrained and unrestrained pregnant m
ice nonexposed to MMC. Combined exposure to 25 mg MMC/kg and restraint
enhanced MMC-induced maternal toxicity, which included deaths and dec
reased body weight gain and food consumption. The number of nonviable
implants was also increased significantly following concurrent exposur
e to MMC (25 mg/kg) and restraint, with the percentage of postimplanta
tion loss increased from 64% (MMC alone) to 100% (MMC plus restraint).
However, the types and incidence of internal and skeletal anomalies o
bserved after administration of 12.5 mg MMC/kg were not increased by m
aternal restraint. These results suggest that maternal stress would en
hance the MMC-induced maternal and embryo/fetal toxicity at doses of M
MC that are highly toxic to the darns, whereas at doses that are less
acutely toxic the role of maternal stress would not be significant.