DETECTION OF SQUAMOUS NEOPLASIA BY FLUORESCENCE IMAGING COMPARING PORFIMER SODIUM FLUORESCENCE TO TISSUE AUTOFLUORESCENCE IN THE HAMSTER-CHEEK POUCH MODEL
I. Pathak et al., DETECTION OF SQUAMOUS NEOPLASIA BY FLUORESCENCE IMAGING COMPARING PORFIMER SODIUM FLUORESCENCE TO TISSUE AUTOFLUORESCENCE IN THE HAMSTER-CHEEK POUCH MODEL, The American journal of surgery, 170(5), 1995, pp. 423-426
BACKGROUND: Early neoplastic changes in the oral cavity may be difficu
lt to detect. Fluorescence imaging using porphyrin-derived drugs has b
een used to enhance detection of neoplasia. Autofluorescence has also
been used for this purpose. This paper compares autofluorescence to po
rfimer sodium-induced fluorescence in the detection of neoplasia in th
e hamster cheek-pouch model. MATERIALS AND METHODS: Neoplasia was indu
ced in the hamster cheek pouch by the application of 9,10-dimethyl-1,2
-benzanthracene. Animals were imaged either with injection of drug (po
rfimer sodium) or without drug (autofluorescence). Imaging was carried
out using a laser-induced fluorescence detection system. Biopies were
performed on imaged sections and histologic grades were assigned. RES
ULTs: Porfimer sodium fluorescence provided 100% sensitivity and speci
ficity in detection of neoplasia. The sensitivity and specificity with
autofluorescence was 76% and 83%, respectively. CONCLUSIONS: Autofluo
rescence provides an accurate means of detecting early neoplastic chan
ges in the hamster cheek-pouch model; however, porfimer sodium imaging
does improve detection rates.