DNA PROTOCOLS FOR TYPING FORENSIC BIOLOGICAL EVIDENCE - CHEMILUMINESCENT DETECTION FOR HUMAN DNA QUANTITATION AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) ANALYSES AND MANUAL TYPING OF POLYMERASE CHAIN-REACTION (PCR) AMPLIFIED POLYMORPHISMS

Methods for identity testing are described that enable extraction of D NA from biological samples, determination of the quantity of human DNA , and genetic analyses of the materials using restriction fragment len gth polymorphism (RFLP) typing and/or amplified fragment length polymo rphism (AMP-FLP) typing of PCR products. The salient features of the p rocedures are simplicity, manual typing, nonradioactive chemiluminesce nt assays or silver staining for detection, and low cost. Most applica tion-oriented laboratories involved in forensic and/or paternity testi ng should be able to implement these procedures.