Ss. Papiha et A. Sertedaki, OLIGONUCLEOTIDE (CAC)(5) FINGERPRINTING - VALIDITY AND RELIABILITY INPATERNITY TESTING, Electrophoresis, 16(9), 1995, pp. 1624-1626
Following the developments of DNA fingerprinting (Jeffreys et al., Nat
ure 1985, 314, 67-73) using the minisatellite probes 33.15 and 33.6, m
any other fingerprinting probes have been described. The wide type M13
phage, 3' HVR region of alpha globin gene, F10 and various synthetic
oligonucleotide probes have been shown to produce multiallelic and ind
ividual specific DNA fingerprints. The two probes developed by Jeffrey
s et al. have been extensively used in forensic and paternity determin
ation in various laboratories, however, for the use of DNA fingerprint
s produced by synthetic oligonucleotide probes, still need critical ev
aluation. In this paper the statistical evaluations of the fingerprint
ing probes (CAC)(5) was carried out by comparing the results produced
by Jeffreys et al., using probes 33.15 and 33.6. The mutation rate cal
culated for (CAC)(5) as 0.015 is nearly similar to the probe 33.15 (0.
011) but higher than the probe 33.6 (0.005). The data obtained using (
CAC)(5) fingerprint in paternity cases from the northeast of England a
re presented.