OLIGONUCLEOTIDE (CAC)(5) FINGERPRINTING - VALIDITY AND RELIABILITY INPATERNITY TESTING

Citation
Ss. Papiha et A. Sertedaki, OLIGONUCLEOTIDE (CAC)(5) FINGERPRINTING - VALIDITY AND RELIABILITY INPATERNITY TESTING, Electrophoresis, 16(9), 1995, pp. 1624-1626
Citations number
6
Categorie Soggetti
Biochemical Research Methods
Journal title
ISSN journal
01730835
Volume
16
Issue
9
Year of publication
1995
Pages
1624 - 1626
Database
ISI
SICI code
0173-0835(1995)16:9<1624:O(F-VA>2.0.ZU;2-F
Abstract
Following the developments of DNA fingerprinting (Jeffreys et al., Nat ure 1985, 314, 67-73) using the minisatellite probes 33.15 and 33.6, m any other fingerprinting probes have been described. The wide type M13 phage, 3' HVR region of alpha globin gene, F10 and various synthetic oligonucleotide probes have been shown to produce multiallelic and ind ividual specific DNA fingerprints. The two probes developed by Jeffrey s et al. have been extensively used in forensic and paternity determin ation in various laboratories, however, for the use of DNA fingerprint s produced by synthetic oligonucleotide probes, still need critical ev aluation. In this paper the statistical evaluations of the fingerprint ing probes (CAC)(5) was carried out by comparing the results produced by Jeffreys et al., using probes 33.15 and 33.6. The mutation rate cal culated for (CAC)(5) as 0.015 is nearly similar to the probe 33.15 (0. 011) but higher than the probe 33.6 (0.005). The data obtained using ( CAC)(5) fingerprint in paternity cases from the northeast of England a re presented.