SOLID-PHASE SYNTHESIS OF A NUCLEOPEPTIDE FROM THE LINKING SITE OF ADENOVIRUS-2 NUCLEOPROTEIN, -SER(P5'CATCAT)-GLY-ASP- - CONVERGENT VERSUS STEPWISE STRATEGY
J. Robles et al., SOLID-PHASE SYNTHESIS OF A NUCLEOPEPTIDE FROM THE LINKING SITE OF ADENOVIRUS-2 NUCLEOPROTEIN, -SER(P5'CATCAT)-GLY-ASP- - CONVERGENT VERSUS STEPWISE STRATEGY, Nucleic acids research, 23(20), 1995, pp. 4151-4161
The synthesis of a nucleopeptide with the sequence -Ser(p(5')CATCAT)-G
ly-Asp- has been undertaken by either convergent or stepwise solid-pha
se strategies, both of which use base-labile permanent protecting grou
ps, The coupling of phosphitylated protected peptides onto oligonucleo
tide-resins did not afford the desired nucleopeptide, which was nevert
heless obtained after oligonucleotide elongation at the hydroxyl group
of the resin-bound peptide and deprotection under mild basic conditio
ns, A preliminary study on the stability of different nucleopeptides t
o bases is also reported.