SOLID-PHASE SYNTHESIS OF A NUCLEOPEPTIDE FROM THE LINKING SITE OF ADENOVIRUS-2 NUCLEOPROTEIN, -SER(P5'CATCAT)-GLY-ASP- - CONVERGENT VERSUS STEPWISE STRATEGY

Citation
J. Robles et al., SOLID-PHASE SYNTHESIS OF A NUCLEOPEPTIDE FROM THE LINKING SITE OF ADENOVIRUS-2 NUCLEOPROTEIN, -SER(P5'CATCAT)-GLY-ASP- - CONVERGENT VERSUS STEPWISE STRATEGY, Nucleic acids research, 23(20), 1995, pp. 4151-4161
Citations number
53
Categorie Soggetti
Biology
Journal title
ISSN journal
03051048
Volume
23
Issue
20
Year of publication
1995
Pages
4151 - 4161
Database
ISI
SICI code
0305-1048(1995)23:20<4151:SSOANF>2.0.ZU;2-N
Abstract
The synthesis of a nucleopeptide with the sequence -Ser(p(5')CATCAT)-G ly-Asp- has been undertaken by either convergent or stepwise solid-pha se strategies, both of which use base-labile permanent protecting grou ps, The coupling of phosphitylated protected peptides onto oligonucleo tide-resins did not afford the desired nucleopeptide, which was nevert heless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditio ns, A preliminary study on the stability of different nucleopeptides t o bases is also reported.