INFLUENCE OF MEMBRANE-COMPONENTS ON THE STABILITY AND DRUG-RELEASE PROPERTIES OF REVERSE-PHASE EVAPORATION VESICLES (REVS) - LIGHT-SENSITIVE ALL-TRANS RETINAL, NEGATIVELY CHARGED PHOSPHOLIPID DICETYLPHOSPHATE AND CHOLESTEROL
M. Gursel et V. Hasirci, INFLUENCE OF MEMBRANE-COMPONENTS ON THE STABILITY AND DRUG-RELEASE PROPERTIES OF REVERSE-PHASE EVAPORATION VESICLES (REVS) - LIGHT-SENSITIVE ALL-TRANS RETINAL, NEGATIVELY CHARGED PHOSPHOLIPID DICETYLPHOSPHATE AND CHOLESTEROL, Journal of microencapsulation, 12(6), 1995, pp. 661-669
Citations number
27
Categorie Soggetti
Pharmacology & Pharmacy","Chemistry Applied","Engineering, Chemical
Incorporation of a negatively charged phospholipid, dicetylphosphate,
initially increased encapsulation efficiency (from 12 to 24%) but beyo
nd 5% (molar) a detrimental effect was observed. Rate of drug release
from REVs was, for most cases, found to be bi-phasic implying partitio
ning between the lipid bilayer and the aqueous compartment. It was not
possible to prepare liposomes with more than 1% (molar) all-trans ret
inal (ATR) as a membrane component. When ATR was reduced to 0.5% (mola
r), encapsulation efficiency increased to 7.76%. Upon exposure to long
wave UV (365 nm), release from ATR containing REVs was increased and
this was attributed to the formation of 13-cis isomer as indicated by
HPLC and UV spectroscopy data.