Aspergillus niger SZ was grown under citric acid accumulation conditio
ns in a 11 bioreactor. Radioactive citric acid was added during the fe
rmentation to the culture medium. The accumulation of radioactive citr
ic acid in the mycellium was measure by measuring radioactive CO2 in t
he offgas. Evidence was obtained of significant turnover and metabolis
m of exogenous citric acid even during the phase of maximum accumulati
on into the medium.