K. Sato et al., DIRECT EFFECT OF CADMIUM ON CITRATE UPTAKE BY ISOLATED RAT RENAL BRUSH-BORDER MEMBRANE-VESICLES, Toxicology letters, 80(1-3), 1995, pp. 161-165
High incidence of multiple kidney stone formation has been observed am
ong workers exposed to cadmium (Cd). Citrate is known to be a protecti
ve factor against renal stone formation. To study the direct effect of
cadmium on citrate uptake by the renal brush border membrane, we expo
sed isolated rat renal brush border membrane vesicles (BBMV) to cadmiu
m and determined their citrate uptake characteristics. BBMV were prepa
red by the divalent cation precipitation method. Citrate uptake was me
asured by the Millipore rapid membrane filtration technique. Preincuba
tion of BBMV with 2 and 10 mM CdCl2 for 1 min significantly inhibited
citrate uptake compared with that of BBMV without Cd. Analysis of the
time course of citrate uptake during 30-min preincubation of BBMV with
0.5 mM Cd also revealed significant reduction of the uptake compared
with that of the control BBMV without preincubation. These findings in
dicate that preincubation of BBMV with cadmium results in time-depende
nt and concentration-dependent inhibition of citrate uptake.