ASSAY OF PENICILLIN ACYLASE IN ORGANIC MEDIA

Authors
ALVES JRS FONSECA LP RAMALHO MT CABRAL JMS
Citation
Jrs. Alves et al., ASSAY OF PENICILLIN ACYLASE IN ORGANIC MEDIA, Biotechnology techniques, 9(10), 1995, pp. 729-730
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
10
Year of publication
1995
Pages
729 - 730
Database
ISI
SICI code
0951-208X(1995)9:10<729:AOPAIO>2.0.ZU;2-4
Abstract
The synthetic substrate 6-nitro-3-(phenylacetamido) benzoic acid (NIPA B) is an appropriate substrate for assaying penicillin acylase activit y in reversed micellar systems of Aerosol - OT in isooctane. Accumulat ion of 6-nitro-3-aminobenzoic acid (NABA) produced by the enzymatic hy drolysis of NIPAB, followed by the increase in absorbance at 405 nm, w as linear at 4 to 20 mM for up to 30 minutes and 15 degrees C to 40 de grees C.