Y. Wang et al., STRATEGIES FOR THE PRODUCTION OF LIPASE BY CANDIDA-RUGOSA - NEURAL ESTIMATION OF BIOMASS AND LIPASE ACTIVITY, Biotechnology techniques, 9(10), 1995, pp. 741-746
Various strategies for the production of lipase by Candida rugosa Didd
ens and Lodder (ATCC 14830) in stirred rank reactors were investigated
. The strain was first screened for lipase production with agar plate
assay based on the fluorescent dye Rhodamine B, followed by adaptation
to the production medium used. The highest lipase activity of 7.6 U/m
l was obtained on a medium containing 40 g olive oil/l and 1 g glucose
/l, with supplying pure oxygen. Neural networks were used in the estim
ation of biomass and lipase activity.