Ca. Rumbley et Ew. Voss, LUPUS-DERIVED AUTOANTIBODIES WITH DUAL AUTOACTIVITY - ANTI-DNA AND ANTI-FC .1. COMPARISON OF IGG AUTOREACTIVITIES WITH SINGLE-CHAIN FV DERIVATIVES, Clinical and experimental immunology, 102(2), 1995, pp. 341-348
Investigations into the intrinsic affinity and reactivity of autoanti-
DNA active sites were initiated through the use of purified monoclonal
IgG and the synthesis of single-chain Fv derivatives of murine monocl
onal anti-DNA autoantibodies BV 04-01 and BV 17-45. Results showed tha
t relative to the respective IgG hybridomas, only the BV 04-01 SCA der
ivative showed demonstrable reactivity with DNA. The monovalent single
-chain derivative of BV 17-45 showed no reactivity with DNA in solutio
n or solid-phase assays, even though the parental IgG had been previou
sly described as high affinity. However, 17-45 displayed reactivity as
a bivalent single-chain derivative. In addition, upon concentration,
BV 17-45 IgG formed a highly stable, papain-resistant precipitate. Inv
estigations into the nature of the precipitate revealed that BV 17-45
possessed significant, DNA-inhibitable autobinding to its own IgG mole
cule. BV 04-01 also possessed similar anti-self reactivity. Thus, both
monoclonal autoantibodies examined in this study possessed dual bindi
ng specificity; anti-DNA and anti-self.