HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF STRUCTURAL ISOMERS USING A CYCLODEXTRIN-POLY(ALLYLAMINE) COATED SILICA COLUMN

High performance liquid chromatography (HPLC) separation of substitute d phenols by isocratic elution was investigated using a beta-cyclodext rin-bonded column. The HPLC support was prepared in-house. This suppor t was based on silica beads coated with a beta-cyclodextrin-containing poly(allylamine). The retention behavior of some substituted phenol i somers and aromatic compounds was studied. The contribution of the ami no groups of the polyamine and of the beta-cyclodextrin cavity to the separation process is discussed. Two retention mechanisms, inclusion c omplex formation and acidbase interaction. were found. The effect of t he composition and pH of the mobile phase on the separation was also e xamined.