STEPWISE GRADIENT THIN-LAYER CHROMATOGRAPHY AND DENSITOMETRIC DETERMINATION OF TAXOL IN EXTRACTS FROM VARIOUS SPECIES OF TAXUS

Authors
MATYSIK G GLOWNIAK K JOZEFCZYK A FURMANOWA M
Citation
G. Matysik et al., STEPWISE GRADIENT THIN-LAYER CHROMATOGRAPHY AND DENSITOMETRIC DETERMINATION OF TAXOL IN EXTRACTS FROM VARIOUS SPECIES OF TAXUS, Chromatographia, 41(7-8), 1995, pp. 485-487
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
41
Issue
7-8
Year of publication
1995
Pages
485 - 487
Database
ISI
SICI code
0009-5893(1995)41:7-8<485:SGTCAD>2.0.ZU;2-I
Abstract
Extracts from Taxus species needles (T. media, T. baccata, T. cuspidat a) were separated on thin layers of silica Si 60 by two-stage developm ent (first stage: 60 % heptane solution of a mixture of 5 % methanol a nd 95 % chloroform; second stage: 70 % heptane solution of the same so lution of methanol in chloroform). The peak of taxol was satisfactoril y separated from the neighbouring peaks to permit its densitometric de termination. Content of taxol for various samples of Taxus species is compared.