COMPARISON OF A MONOCLONAL ANTIBODY-BASED IGM CAPTURE ELISA WITH A NEUTRALIZATION ASSAY FOR ASSESSING RESPONSE TO TRIVALENT ORAL POLIOVIRUSVACCINE

Monoclone-based IgM capture ELISAs were developed for each of the thre e poliovirus serotypes and compared with a neutralization assay for de tecting response to trivalent oral poliovirus vaccine among 224 infant s. The IgM-based response rates were significantly higher than the neu tralizing antibody-based rates: 958 versus 83% to poliovirus type 1, 9 9% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus t ype 3. IgM responses to the first vaccine dose were significantly asso ciated between serotypes, suggesting that some of the discordance may reflect a heterotypic IgM response. When the response rates in 4 vacci ne formulation groups were compared, group differences using the two a ssays were similar for poliovirus types I and 2 but not for type 3. Th erefore, IgM results using these assays may not be adequate substitute s for neutralizing antibody results when determining vaccine response.