FASTER AND CHEAPER PCR ON A STANDARD THERMOCYCLER

The FCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the tim e of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for man y types of analysis. The PCR can be done even in a reaction volume as low as 1 mu l. The recommended volume, 2.5 mu l or 5 mu l, allows sign ificant savings in the laboratory budget especially for laboratories w hich use PCR frequently and on a large scale.