Sustained genetic advances requires that genetically diverse parents b
e mated to form segregating populations for selection. Genetic diversi
ty of U.S. cotton (Gossypium hirsutum L.) cultivars has not been exten
sively quantified. We assessed diversity among 126 Upland cotton culti
vars release between 1980 and 1990 by use of coefficient of parentage
(CP). In computing CP, we utilized assumptions appropriate for self-po
llinated crops. Mean CP among the 126 cultivars was 0.07, implying a g
enetically diverse group. However, cluster analysis revealed 12 distin
ct gene pools, with mean within-cluster CP = 0.25 and between-cluster
origin. The CP analysis indicates that Acala-type cultivars are more d
iverse that those bred in the Mississippi Delta or southeastern USA. A
trend in germplasm usage in the late 1980s was the repeated mating of
genetically related material, or reselection within germplasm, to dev
elop proprietary cultivars. To ensure continued progress in cotton imp
rovement, we suggest that cotton breeders consider the pedigree of par
ents prior to population synthesis.