The advertisement calls of 23 Bolivian anuran species in the family Le
ptodactylidae are described including information on calling behaviour
. For each species, a characteristic audiospectrogram and oscillogram
are presented. Numerical information about the spectral and temporal f
eatures of the calls is provided and, when available, the results are
compared with any previous description of the calls. A phenogram based
on holistic comparisons of the mating calls (audiospectrogram correla
tion) is presented for the genera Eleutherodactylus, Leptodactytus, an
d Physalaemus. The intrageneric variability of four basic call paramet
ers is compared. The results suggest that fundamental and dominant fre
quency (and possibly duration for the genus Physalaemus) are the most
likely variables to play a role in isolation of congeneric species.