APOLIPOPROTEINS E3 AND E4 INDUCE, AND TRANSTHYRETIN PREVENTS ACCUMULATION OF THE ALZHEIMERS BETA-AMYLOID PEPTIDE IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS

Cultured brain vascular smooth muscle cells (SMCs) accumulate beta-pep tide in intracytoplasmic granules [9,10,33]. We show here that apoE3 a nd E4 induces the intracytoplasmic beta-peptide accumulation in cultur ed human and canine SMCs. The induction is dose-dependent and the accu mulated granules also contain apoE and some were thioflavine S-positiv e. The deposits induced with apoE3 were more abundant though less stab le than with apoE4. Transthyretin at physiological concentrations bloc ked the effects of apoE3/E4. Thus, accumulation of beta-peptide appear s to be regulated by beta-peptide carrier proteins.