The temperature-phased anaerobic biofilter (TPAB) process is a new hig
h-rate anaerobic treatment system that includes a thermophilic biofilt
er connected in series to a mesophilic biofilter providing for two-tem
perature, two-phase treatment. Three TPAB systems with thermophilic:me
sophilic reactor size ratios of 1:7, 1:3, and 1:1 were operated at sys
tem hydraulic retention times (HRTs) of 24, 36, and 48 hours at temper
atures of 56 degrees C in the thermophilic phase and 35 degrees C in t
he mesophilic phase. The three TPAB systems achieved soluble and total
chemical oxygen demand (GOD) reductions in excess of 97% and 90%, res
pectively, for a synthetic milk substrate over a range of system COD l
oadings from 2 to 16 g COD/L/d. There was little difference in perform
ance between the three TPAB systems based on COD reduction and methane
production, making it feasible to use a smaller thermophilic first ph
ase in the TPAB system. At the 48-hour system HRT, the 6-hour thermoph
ilic phase biofilter reached saturation loading at a COD load of 48 g/
L/d. After further increases in organic loading, the methane productio
n decreased in the thermophilic first phase and increased in the corre
sponding mesophilic second phase while levels of n-valeric and butyric
acids increased significantly. It is proposed that a microbial popula
tion shift occurred in the thermophilic first phase at high organic lo
adings at low HRTs. Although a decrease in methane production from the
thermophilic phase was observed, the performance of the overall two-s
tage TPAB system did not decline. The TPAB systems were observed to ou
tperform single-stage anaerobic filters operated at equivalent HRTs an
d organic loadings.