Biological solids have significant potential for sorption of soluble o
rganic matter from wastewater streams. The anaerobic biosorption proce
ss uses the sorption capabilities of active biomass for the treatment
of wastewater. Biosorption is a rapid process. Approximately 40% remov
al of organic matter was obtained in only 15 minutes after the substra
te was brought into contact with the biomass. Anaerobic biomass was gr
own in 10-L source reactor; operated at a constant temperature of 35 d
egrees C. Biosorption experiments were performed with active biomass i
n 2-L batch reactors using synthetic milk as a substrate. The effects
of mixing times, temperature, substrate concentration, biomass concent
ration, and granular biomass particle size were investigated. Temperat
ure studies indicated increased uptake at higher temperature than at l
ower temperature. It was observed that wastes at temperatures as low a
s 7 degrees C could be biosorbed without the application of external h
eat. Higher removals were obtained with the small granular biomass tha
n with the large granules. Increasing the biomass concentration result
ed in an increase in the percentage removal of organic matter. The res
ults were expressed in terms of adsorption isotherms.