Fermented organic wastes were investigated as electron donors for deni
trification in activated sludge. Fermentation of primary domestic wast
ewater sludge improved denitrification rates in nonacclimated activate
d sludge, whereas fermentation of wastewater had no effect. In both ac
climated and nonacclimated activated sludges, higher denitrification r
ates were obtained with fermented sludges (from domestic wastewater or
swine waste) than with either methanol or acetate. Methanol was equiv
alent to acetate as electron donor for denitrification in acclimated s
ludges but supported lower denitrification rates than acetate in nonac
climated sludges. Mixing and pH were important determinants of volatil
e fatty acid yields from organic wastes, whereas temperature in the ra
nge of 28 degrees C to 35 degrees C had little influence.