C. Ostermeier et al., F-V FRAGMENT MEDIATED CRYSTALLIZATION OF THE MEMBRANE-PROTEIN BACTERIAL CYTOCHROME-C-OXIDASE, Nature structural biology, 2(10), 1995, pp. 842-846
Crystallization of membrane proteins, a prerequisite for their X-ray c
rystallographic analysis, remains difficult. Here, we demonstrate that
the crystallization of the cytochrome c oxidase from Paracoccus denit
rificans can be mediated by co-crystallization with an antibody F-v fr
agment. The crystals obtained contain all-four subunits of this membra
ne protein complex and the F-v fragment. The approach of co-crystalliz
ing membrane proteins with antibody fragments should be useful in obta
ining well-ordered crystals of membrane proteins in general.