COMPARATIVE GENOMIC HYBRIDIZATION OF FORMALIN-FIXED, PARAFFIN-EMBEDDED BREAST-TUMORS REVEALS DIFFERENT PATTERNS OF CHROMOSOMAL GAINS AND LOSSES IN FIBROADENOMAS AND DIPLOID AND ANEUPLOID CARCINOMAS
T. Ried et al., COMPARATIVE GENOMIC HYBRIDIZATION OF FORMALIN-FIXED, PARAFFIN-EMBEDDED BREAST-TUMORS REVEALS DIFFERENT PATTERNS OF CHROMOSOMAL GAINS AND LOSSES IN FIBROADENOMAS AND DIPLOID AND ANEUPLOID CARCINOMAS, Cancer research, 55(22), 1995, pp. 5415-5423
Comparative genomic hybridization serves as a screening test for regio
ns of copy number changes in tumor genomes. We have applied the techni
que to map DNA gains and losses in 33 cases of formalin-fixed, paraffi
n-embedded primary breast tumors (13 fibroadenomas and 10 diploid and
10 aneuploid carcinomas). No genomic imbalances were found in fibroade
nomas. Recurrent findings in adenocarcinomas include copy number incre
ases for chromosomes 1q (14 of 20 samples), 8q (10 of 20), 17q (5 of 2
0), 6p (3 of 20), 13q (3 of 20), and 16p (3 of 20), and copy number de
creases for chromosomes 22 (7 of 20), 17p (6 of 20), and 20 (3 of 20).
Regional high level copy number increases were observed on chromosome
bands 1q32, 8p11, 8q24, 10p, 11q13, 12p, 12q15, 17q11-12, and 17q22-2
4. The majority of the samples were studied for gene amplification of
c-myc, c-erbB2, cycD1, and int-2 by means of Southern blot analysis. T
he comparison with DNA ploidy measurements revealed a different distri
bution and a significantly higher number of chromosomal