SEPARATION OF TAXOL AND CEPHALOMANNINE BY COUNTERCURRENT CHROMATOGRAPHY

Authors
CHIOU FY KAN P CHU IM LEE CJ
Citation
Fy. Chiou et al., SEPARATION OF TAXOL AND CEPHALOMANNINE BY COUNTERCURRENT CHROMATOGRAPHY, Journal of liquid chromatography & related technologies, 20(1), 1997, pp. 57-61
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of liquid chromatography & related technologies → ACNP
ISSN journal
10826076
Volume
20
Issue
1
Year of publication
1997
Pages
57 - 61
Database
ISI
SICI code
1082-6076(1997)20:1<57:SOTACB>2.0.ZU;2-M
Abstract
Countercurrent chromatography was used to separate taxol from cephalom annine, on a preparative scale for the final purification of taxol. A solvent system consisting of hexane:ethyl acetate:methanol: ethanol:wa ter in 5:7:5:1:6.5 was found to be suitable in achieving near base-lin e resolution of the two components. It was found that the amount of me thanol in the solvent system was very critical to a successful separat ion operation.