IMMOBILIZED IRON(III) METAL AFFINITY-CHROMATOGRAPHY FOR THE SEPARATION OF PHOSPHORYLATED MACROMOLECULES - LIGANDS AND APPLICATIONS

This review highlights the advances in Fe(III)-Immobilized Metal Affin ity Chromatography [Fe(III)-IMAC] for the separation and characterizat ion of phosphorylated biomolecules. Fe(III)-IMAC has demonstrated a se lective interaction with phosphate groups and other hard Lewis bases s uch as carboxylate and phenol functional groups. These properties make this technique useful for separating and studying macromolecules. Sin ce phosphorylation is a central means of regulation in eukaryotes and prokaryotes, the development of Fe(III)-IMAC is important. Various iro n chelating ligands and their properties are discussed. General featur es of Fe(III)-IMAC are examined and examples of useful separations are considered.