A compact and low cost bench-top, pulsed electric field treatment syst
em was designed and developed. The unit consisted of a high-voltage pu
lse generator (less than or equal to 30 kV) and a treatment chamber wi
th less than or equal to 148 mi capacity. Over the set-lip voltage ran
ge of 4-26 kV, 30 pulses (with instant charge reversal) were applied t
o eight different enzyme solutions using a 0.3-cm electrode distance,
a 13-87 kV/cm field, 0.5-Hz pulse frequency, 2-mu s pulse width and 20
degrees C process temperature. For some enzymes, activities were redu
ced after the pulse treatments: lipase, glucose oxidase and heat-stabl
e Lx-amylase exhibited a vast reduction of 70-85%; peroxidase and poly
phenol oxidase showed a moderate 30-40% reduction whereas alkaline pho
sphatase only displayed a slight 5% reduction under the conditions emp
loyed. On the other hand the enzyme activities of lysozyme and pepsin
were increased under a certain range of voltages. Electric pulse profi
le (instant charge reversal) played a very important role in reducing
the activities of various enzymes. (C) 1997 Elsevier Science Limited.
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