VIBRIO TRACHURI SP-NOV, A NEW SPECIES ISOLATED FROM DISEASED JAPANESEHORSE MACKEREL

A new species, Vibrio trachuri sp, nov., was isolated from the culture d Japanese horse mackerel (Trachurus japonicus). These Vibrio were Gra m negative, motile rods and formed yellow colonies on BTB teepol and T CBS plate, turned TSI medium to yellow and was sensitive to 150 mu M O /129 (2,4-diamino-6,7-diisopropyl pteridine phosphate) like Listonella anguillarum which has been described as Vibrio anguillarum, However, the results of VP test and decarboxylation of lysine or dihydrolation of arginine suggested that these Vibrio are rather closely related to V. parahaemolyticus. DNA similarity determined by the microplate hybri dization technique revealed that these Vibrio are genetically quite di stant from Listonella anguillarum or V; parahaemolyticus and rather cl ose to V. harveyi, although there was no Vibrio species which had more than 70% similarity value. From these results we propose to nominate Vibrio trachuri sp, nov. for this new Vibrio species.