Cr. Lacroix et J. Macintyre, NEW TECHNIQUES AND APPLICATIONS FOR EPI-ILLUMINATION LIGHT-MICROSCOPY, Canadian journal of botany, 73(11), 1995, pp. 1842-1847
This modification to the technique of epi-illumination light microscop
y makes use of a new system of lenses that replaces expensive and not
readily available dipping cone objectives. The newer objectives offer
at least comparable resolution and depth of field, along with simple p
reparation procedures. An epi-illumination system is a good intermedia
te between the stereo microscope and a scanning electron microscope, o
ffering magnification at high power that can aid in evaluation of pote
ntial scanning electron microscope specimens, as well as the time- and
material-saving feature of being able to eliminate unsuitable scannin
g electron microscope specimens.