IMMUNOHISTOCHEMICAL DISTRIBUTION OF SURFACTANT APOPROTEINS IN HYPOPLASTIC LUNGS OF NONIMMUNOLOGICAL HYDROPS-FETALIS

Forty-three patients with nonimmunologic hydrops fetalis (NIHF), inclu ding 32 patients (74%) with hypoplastic lung, were immunohistochemical ly examined for the expression of surfactant apolipoproteins (SPs), us ing anti-gamma G immunoglobulins against human SP-A with a molecular w eight (MW) of 35 It and SP-B with a MW of 5 K compared with that in 59 patients in a control group and 45 patients with hypoplastic lung ind uced by causes other than NIHF. In the control group, SP-A was express ed in the lungs from 23 gestational weeks and became more numerous and intense in alveolar type II cells after 31 gestational weeks, whereas SP-B began to be expressed from 20 gestational weeks, and almost all patients showed a diffuse positivity after 26 gestational weeks. In th e NIHF group, SP-A expression was generally weak, even after 31 gestat ional weeks. Moreover, most of the patients showing a weak expression of SP-A were also associated with hypoplastic lung and had a clinical history persistent intrauterine pleural effusion of more than 2 weeks. Conversely, the immunoreactivity of SP-B was well preserved in NIHF c ases either with or without hypoplastic lung. These results suggest th at in the NMF lung, there is a possible delay in the functional matura tion or development of SP-A synthesis by alveolar type II cells, and t his retardation of the functional maturation in type II cells also par ticipates in the postnatal respiratory insufficiency in NIHF. Copyrigh t (C) 1995 by W.B. Saunders Company