T. Jelinek et al., GEOGRAPHIC DIFFERENCES IN THE SENSITIVITY OF A POLYMERASE CHAIN-REACTION FOR THE DETECTION OF PLASMODIUM-FALCIPARUM INFECTION, The American journal of tropical medicine and hygiene, 55(6), 1996, pp. 647-651
Citations number
12
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
The amplification of target DNA by highly specific probes using the po
lymerase chain reaction (PCR) provides a highly sensitive and specific
method for the detection of malaria infection. The use the of PCR in
settings with varying endemicity within one survey area has not been i
nvestigated intensively. Therefore, a cross-sectional study was conduc
ted in the districts of Kabarole and Bundibugyo in western Uganda usin
g material from three villages with different epidemiologic situations
regarding malaria and DNA primers for a PCR that had shown satisfacto
ry sensitivity and specificity in previous trials. The sensitivity of
the PCR varied significantly (P < 0.001) in the three survey Villages
(between 63.2% and 83.9% for the primer pair K1-14-1 and between 37.9%
and 69.9% for the primer pair MSP-1) and was highly linked to geograp
hic differences and social exchanges of the inhabitants with other are
as of the district. According to the results of this investigation, it
is advisable not to use a single primer pair in epidemiologic field s
tudies for the detection of falciparum malaria. The use of combined pr
imer pairs and the frequent confirmation of the results by microscopy
are recommended.