GAK - A CYCLIN-G ASSOCIATED KINASE CONTAINS A TENSIN AUXILIN-LIKE DOMAIN/

We have cloned a cDNA encoding a novel association partner of cyclin G by West-Western blotting, The cDNA encodes a protein that harbors a S er/Thr protein kinase-like catalytic domain at the N-terminal, Hence, we named it GAK (cyclin (G) under bar-(a) under bar ssociated (k) unde r bar inase). The long C-terminal extension shares homology with tensi n and auxilin, and contains a leucine zipper region, Co-immunoprecipit ation and Western blotting showed that GAK and cyclin G associate toge ther in vivo, GAK also co-precipitated with CDK5, and CDK5 was found t o be associated with cyclin G, We also showed by BIAcore analysis that the GAK-cyclin G interaction was direct.