INCREASE IN TOTAL PROTEIN FOLLOWING INFECTION OF CV-1 CELLS WITH SV40VIRUS AS ASSAYED BY FLOW-CYTOMETRY

The changes in cell size and total protein were determined for G1-arre sted, contact-inhibited CV-1 cells infected with Simian virus 40 (SV40 ). The assays used were the Biorad total protein assays (Bradford and DC protein assays) on a standard number of cells, total protein as ass ayed by fluorescein isothiocyanate (FITC) and SR101 by flow cytometry, orthoganol (90 degrees) light scatter by flow cytometry, and direct m icroscopic measurement with an ocular micrometer. Uninfected CV-1 cell s and two cell lines with variations in DNA content (diploid vs. tetra ploid) were used as controls for the studies presented. The results de monstrated a 40-60% increase in total protein at 32 to 42 h postinfect ion. These increases were similar to values obtained as control cells progress through the cell cycle. At later times postinfection (>42 h), total protein decreased due to cellular changes resulting from viral replication and cell death.