GENETIC-TRANSFORMATION OF WHEAT (TRITICUM -AESTIVUM L) BY AGROBACTERIUM-TUMEFACIENS

Genetic transformation. of spring, common wheat (Triticum aestivum L.) using Agrobacterium tumefaciens was performed. Agrobacterium strain C 158, which contains the cointegrate of plasmids pCV2260 and pSIR42, wa s used. Agrobacterium cells were applied to flower pistils after artif icial pollination. The seed set was 3%. F-1 plants were morphologicall y similar to the control. Genomic DNA analysis by dot hybridization an d PCR confirmed the existence of recombinant DNA in some plants. F-2 w as obtained from the transformed plants; these plants also contained f oreign DNA. Repeated transformation by this method gave the same resul t. Transformation frequency was at least 2.7%.