INVESTIGATION OF THE ACTINOBACILLUS-ACTINOMYCETEMCOMITANS GENOME BY PULSED-FIELD GEL-ELECTROPHORESIS

Authors
VALCARCEL J ALLARDETSERVENT A BOURG G OCALLAGHAN D MICHAILESCO P RAMUZ M
Citation
J. Valcarcel et al., INVESTIGATION OF THE ACTINOBACILLUS-ACTINOMYCETEMCOMITANS GENOME BY PULSED-FIELD GEL-ELECTROPHORESIS, Oral microbiology and immunology, 12(1), 1997, pp. 33-39
Citations number
38
Categorie Soggetti
Immunology,Microbiology,"Dentistry,Oral Surgery & Medicine
Journal title
Oral microbiology and immunology → ACNP
ISSN journal
09020055
Volume
12
Issue
1
Year of publication
1997
Pages
33 - 39
Database
ISI
SICI code
0902-0055(1997)12:1<33:IOTAGB>2.0.ZU;2-P
Abstract
Pulsed field gel electrophoresis was used to investigate nineteen stra ins of Actinobacillus actinomycetemcomitans. The genome was found to c ontain a single chromosome whose size we estimate to be 2300 kb from t he sum of restriction fragments generated with rare cutting endonuclea ses. We detected the presence of large plasmids with sizes ranging fro m 35 to 300 kb. In some strains, extrachromosomal elements constitute over 20% of the total genome. Comparison of the profiles of ApaI diges ts of the 19 strains showed a high degree of polymorphism with 13 diff erent profiles, providing a new tool for epidemiological studies.