APPLICATION OF SEPHAROSE AND SEPHADEX MODIFIED BY MEANS OF RADIATION GRAFTING IN SEPARATION OF BIOMOLECULES

In this work Sepharose-NASI-BSA and Sephadex-NASI-BSA were prepared by means of preirradiation grafting and direct radiation grafting separa tely. The radiation grafting condition were given. In order to get tho se compounds the conjugation reaction between NASI and BSA was investi gated as well. The reaction was performed at 37 degrees C for 2hs, but in the case of Sephadex-NASI, it took longer time, 6hs, to finish the conjugation reaction. The application of the radiation modified matri xes as a kind of filling materials of column chromatography in separat ion of D,L-tryptophans was studied The results showed that this method was accessible after some improvements.